Epigenetics Boot Camp: Planning and Analyzing DNA Methylation Studies for Human Populations
The Epigenetics Boot Camp is a two-day intensive boot camp of seminars and hands-on analytical sessions to provide an overview of concepts, techniques, and data analysis methods utilized in human epigenetics studies and focused on DNA methylation array.
This two-day intensive boot camp integrates the principle concepts of epigenetics and the effects of risk factors on the epigenome as we step through the key components of designing and executing DNA methylation studies. Led by a team of scientists with more than 50 years of combined expertise in the field of environmental epigenetics, this epigenetics training will integrate seminar lectures with hands-on computer sessions to put concepts into practice. Emphasis will be given to leveraging existing resources from ongoing studies and initiating new investigations. The afternoon lab sessions will provide an opportunity to work hands-on with real methylation data generated by the Illumina Infinium MethylationEPIC (850k) BeadChip platform. Participants will learn and practice data handling, cleaning, and basic analysis of epigenome-wide DNA methylation data. This training will not cover WGBS or analyses from sequencing experiments in animal models.
By the end of the epigenetics training, participants will be familiar with the following topics:
Principles of epigenetics
Concept ideation and study design
Sample collection and processing
Laboratory methods and platforms for analysis
Data handling and cleaning
Analysis of 450K/EPIC data
Study design advantages and pitfalls
Emerging epigenetic topics and laboratory platforms
Investigators at all career stages are welcome to attend, and we particularly encourage trainees and early-stage investigators to participate.
PREREQUISITES AND REQUIREMENTS
Each participant is required to have a personal laptop/computer and a free, basic RStudio Cloud account. All lab sessions will be done using RStudio Cloud.
The Boot Camp will use data sets in R, therefore participants must have a basic understanding of this platform prior to attending the Boot Camp. If you have never used this platform before, make sure to step through all the tutorials outlined below so you have the basic skills needed for Boot Camp success.